rat vglut3  (Alomone Labs)

Journal: eNeuro

Article Title: Slow NMDA-Mediated Excitation Accelerates Offset-Response Latencies Generated via a Post-Inhibitory Rebound Mechanism

doi: 10.1523/ENEURO.0106-19.2019

Figure Lengend Snippet: Primary and secondary antibodies used for immunocytochemistry

Article Snippet: VGLUT3 , Peptide (C)ELNHEAFVSPRKK, corresponding to amino acid residues 533–545 of rat VGLUT3 (accession Q7TSF2); cytoplasmic, C terminus , Alomone Labs , AGC-037 , Rabbit , 1:300.

Techniques: Clone Assay, Recombinant, Purification, Isolation, Strep-tag

Journal: eNeuro

Article Title: Slow NMDA-Mediated Excitation Accelerates Offset-Response Latencies Generated via a Post-Inhibitory Rebound Mechanism

doi: 10.1523/ENEURO.0106-19.2019

Figure Lengend Snippet: Histochemical profile of excitatory and inhibitory inputs to the SPN. A , B , D , E , G , H , Glycinergic input forms the most prominent input to SPN (white dotted circle in A , D , G ) and is depicted by neuronal GlyT2 (green) labeling. Immunolabeling for microtubule associated protein 2 (MAP2, blue) is used as a neuronal marker in all images. Glutamatergic inputs are shown by labeling for the VGLUTs (magenta): VGLUT1 ( A – C ), VGLUT2 ( D – F ), VGLUT3 ( G – I ). A–F , While both VGLUT1 ( B , white arrows) and VGLUT2 ( E , white arrows) positive synaptic boutons are present at the soma, VGLUT2 boutons are also seen in the neuropil ( E , white arrowheads). G–I , VGLUT3 shows only weak somatic but no presynaptic bouton labeling. Scale bars = 200 µm (left images) and 20 µm (middle and right images).

Article Snippet: VGLUT3 , Peptide (C)ELNHEAFVSPRKK, corresponding to amino acid residues 533–545 of rat VGLUT3 (accession Q7TSF2); cytoplasmic, C terminus , Alomone Labs , AGC-037 , Rabbit , 1:300.

Techniques: Labeling, Immunolabeling, Marker

Journal: Frontiers in Neuroanatomy

Article Title: Dopamine in the auditory brainstem and midbrain: co-localization with amino acid neurotransmitters and gene expression following cochlear trauma

doi: 10.3389/fnana.2015.00088

Figure Lengend Snippet: Antibodies.

Article Snippet: VGLUT3 Amino acids 546–588 of rat VGLUT3 , Mouse/Clone N34/34 , NeuroMab, Davis, CA 75-073 , 1:100 , .

Techniques: Recombinant, Purification

Journal: Frontiers in Neuroanatomy

Article Title: Dopamine in the auditory brainstem and midbrain: co-localization with amino acid neurotransmitters and gene expression following cochlear trauma

doi: 10.3389/fnana.2015.00088

Figure Lengend Snippet: Dopamine and AAN related genes are differentially expressed in the inferior colliculus (IC). Changes were assessed in DA related (A) , inhibitory (B,C) , and excitatory (D) AAN related gene expression in the IC 3 days and 2 months following bilateral cochlear ablation. TH expression decreased significantly in the IC at both 3 days (31%) and 2 months (38%). In addition to the DA receptor subunit, Drd1A (15%-3 days; 23%-2 months; A ), four AAN related genes showed the same pattern of expression as TH: significant decreases at both time points. Of these, two were related to excitatory neurotransmission (VGLUT2: 60%-3 days and 32%-2 months; VGLUT3: 62%-3 days and 52%-2 months) and two related to inhibitory neurotransmission (GABAtp: 15%-3 days and 22%-2 months; GAD67: 29%-3 days and 36%-2 months). Interestingly, seven genes were significantly decreased (Drd2-30%; Drd5:-18%; GLYT1- 31%; GLYT2- 45%; GAD65- 22%; GABArb1- 26%) only at the 2 months time point (A,C,D) . Error bars: SD; asterisks: p ≤ 0.05.

Article Snippet: VGLUT3 Amino acids 546–588 of rat VGLUT3 , Mouse/Clone N34/34 , NeuroMab, Davis, CA 75-073 , 1:100 , .

Techniques: Gene Expression, Expressing

Journal: Frontiers in Neuroanatomy

Article Title: Dopamine in the auditory brainstem and midbrain: co-localization with amino acid neurotransmitters and gene expression following cochlear trauma

doi: 10.3389/fnana.2015.00088

Figure Lengend Snippet: In the IC VGLUT3 is expressed and produced. Gene expression for VGLUT3 in the IC was demonstrated using PCR (A) , with a predicted band of approximately 1.9 kb. Western blotting for VGLUT3 protein in the IC resulted in a single band ∼65 kDa (B) .

Article Snippet: VGLUT3 Amino acids 546–588 of rat VGLUT3 , Mouse/Clone N34/34 , NeuroMab, Davis, CA 75-073 , 1:100 , .

Techniques: Produced, Gene Expression, Western Blot

Journal: Frontiers in Neuroanatomy

Article Title: Dopamine in the auditory brainstem and midbrain: co-localization with amino acid neurotransmitters and gene expression following cochlear trauma

doi: 10.3389/fnana.2015.00088

Figure Lengend Snippet: In the IC VGLUT3 is distributed throughout each subdivision. Although some labeling is observed in terminals, the majority of labeling for VGLUT3 is contained within somata and dendrites (A–C) . In the CNIC large and small multipolar neurons with round somata and proximal dendrites are labeled for VGLUT3 (A,A’) . While prominent somatic labeling for VGLUT3 is found in the ECIC (B,B’) and DCIC (C,C’) localized to small elongated neurons with little to no labeling of proximal dendrites (arrowheads) as well as within larger neurons, including proximal dendrites (arrows). CNIC – central nucleus of the IC; ECIC – external cortex of the IC; DCIC – dorsal cortex of the IC; Scale bars: 20 μm.

Article Snippet: VGLUT3 Amino acids 546–588 of rat VGLUT3 , Mouse/Clone N34/34 , NeuroMab, Davis, CA 75-073 , 1:100 , .

Techniques: Labeling

Journal: Frontiers in Neuroanatomy

Article Title: Dopamine in the auditory brainstem and midbrain: co-localization with amino acid neurotransmitters and gene expression following cochlear trauma

doi: 10.3389/fnana.2015.00088

Figure Lengend Snippet: In the DCIC VGLUT3 (green) and TH (red) are co-localized in somata and dendrites (A–F). In the DCIC double labeling for VGLUT3 and TH was primarily observed in somata and dendrites. Arrows indicate somatic and dendritic labeling for VGLUT3 and TH. The asterisks indicate neurons in the DCIC labeled only for VGLUT3. DCIC – dorsal cortex of the IC; Scale bars: 10 μm.

Article Snippet: VGLUT3 Amino acids 546–588 of rat VGLUT3 , Mouse/Clone N34/34 , NeuroMab, Davis, CA 75-073 , 1:100 , .

Techniques: Labeling

Journal: Frontiers in Neuroanatomy

Article Title: Dopamine in the auditory brainstem and midbrain: co-localization with amino acid neurotransmitters and gene expression following cochlear trauma

doi: 10.3389/fnana.2015.00088

Figure Lengend Snippet: Gene expression and immunocytochemistry comparison in the cochlear nucleus (CN).

Article Snippet: VGLUT3 Amino acids 546–588 of rat VGLUT3 , Mouse/Clone N34/34 , NeuroMab, Davis, CA 75-073 , 1:100 , .

Techniques: Gene Expression, Immunocytochemistry, Comparison

Journal: Frontiers in Neuroanatomy

Article Title: Dopamine in the auditory brainstem and midbrain: co-localization with amino acid neurotransmitters and gene expression following cochlear trauma

doi: 10.3389/fnana.2015.00088

Figure Lengend Snippet: Gene expression and immunocytochemistry summary in the inferior colliculus (IC).

Article Snippet: VGLUT3 Amino acids 546–588 of rat VGLUT3 , Mouse/Clone N34/34 , NeuroMab, Davis, CA 75-073 , 1:100 , .

Techniques: Gene Expression, Immunocytochemistry

Journal:

Article Title: Immunohistochemical evidence for synaptic release of glutamate from orexin terminals in the locus coeruleus

doi: 10.1016/j.neuroscience.2010.06.003

Figure Lengend Snippet: Primary antibodies

Article Snippet: No homology with other proteins 12 VGluT3 GP Millipore AB5421 Synthetic peptide from cloned rat VGluT3 protein 9 (AA 569 – 588) The AB labels VGluT3+ cells and fibers, in agreement with other VGluT3 antisera 10 VGluT3 Rb SY SY 135 203 Recombinant C-terminus of mouse VGLUT 3 (AA 543 – 601) 12 Specific for mouse VGLUT 3.

Techniques: Sequencing, Immunostaining, Staining, Purification, Recombinant, Strep-tag, Clone Assay, Control, Immunopeptidomics

Journal:

Article Title: Immunohistochemical evidence for synaptic release of glutamate from orexin terminals in the locus coeruleus

doi: 10.1016/j.neuroscience.2010.06.003

Figure Lengend Snippet: Combinations for dual-immunostaining with primary antibodies 1

Article Snippet: No homology with other proteins 12 VGluT3 GP Millipore AB5421 Synthetic peptide from cloned rat VGluT3 protein 9 (AA 569 – 588) The AB labels VGluT3+ cells and fibers, in agreement with other VGluT3 antisera 10 VGluT3 Rb SY SY 135 203 Recombinant C-terminus of mouse VGLUT 3 (AA 543 – 601) 12 Specific for mouse VGLUT 3.

Techniques:

Journal:

Article Title: Immunohistochemical evidence for synaptic release of glutamate from orexin terminals in the locus coeruleus

doi: 10.1016/j.neuroscience.2010.06.003

Figure Lengend Snippet: Fluorescent images of Orx/VGluT1, VGluT2, VGluT3 or VGAT dual-immunostaining in LC. (A-D) Orx+ varicosities (as labeled with Gt-anti-Orx-A primary and Cy5-conjugated secondary antibodies, A1, B1, C1 and D1), which were considered as small (less than approximately 0.5 µm in diameter, small solid arrowheads) or large (>0.5 µm diameter, large solid arrowheads), were examined for double-labeling for the vesicular transporters in fluorescence microscopy. Note all Orx+ varicosities were judged negative (open arrowheads) for VGluT1 (A2, labeled with the Rb-anti-VGluT1 primary and Cy2-conjugated secondary antibodies), VGluT3 (C2, labeled with the Rb-anti-VGluT3 primary and Cy2-conjugated secondary antibodies) and VGAT (D2, labeled with the Rb-anti-VGAT primary and Cy2-conjugated secondary antibodies). Although one small and one large Orx+ varicosity (B1) are also negative for VGluT2 (B2, open arrowheads, labeled with the GP-anti-VGluT2 primary and Cy2-conjugated secondary antibodies), one large Orx+ varicosity (B1) is positive for VGluT2 (B2, solid arrowhead). Note also that among the VT stained varicosities (indicated by small arrows for Orx-/VT+), VGluT2+ varicosities are most numerous among the VGluT+ and similar in density to the VGAT+ varicosities in the LC. Scale bar = 2 µm.

Article Snippet: No homology with other proteins 12 VGluT3 GP Millipore AB5421 Synthetic peptide from cloned rat VGluT3 protein 9 (AA 569 – 588) The AB labels VGluT3+ cells and fibers, in agreement with other VGluT3 antisera 10 VGluT3 Rb SY SY 135 203 Recombinant C-terminus of mouse VGLUT 3 (AA 543 – 601) 12 Specific for mouse VGLUT 3.

Techniques: Immunostaining, Labeling, Fluorescence, Microscopy, Staining

Journal: The Journal of Neuroscience

Article Title: Inputs Underlying the ON–OFF Light Responses of Type 2 Wide-Field Amacrine Cells in TH::GFP Mice

doi: 10.1523/JNEUROSCI.6235-10.2011

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: VGluT3 , Synthetic peptide from rat VGluT3 protein , Millipore, AB5421 , 1:5000, guinea pig, polyclonal.

Techniques: Sequencing, Recombinant, Binding Assay

Journal: The Journal of Neuroscience

Article Title: Inputs Underlying the ON–OFF Light Responses of Type 2 Wide-Field Amacrine Cells in TH::GFP Mice

doi: 10.1523/JNEUROSCI.6235-10.2011

Figure Lengend Snippet: Type 2 cells receive inputs from VGluT3-immunoreactive amacrine cells and express glycine receptors. A–D, Single scans (0.2 μm) of a retina section from a TH::GFP mouse labeled with antibodies against VGluT3, a marker for a certain type of glycinergic amacrine cell (B), and the glycine receptor 2α subunit (C). D, Merged image. E, Projection of 15 scans (3 μm). F–J, Single confocal scans show the area marked in D under higher magnification. GFP-positive dendrites from type 2 cells (E) are contacted by VGluT3-positive dendrites (G). GlyR2α-positive puncta (H) are visible at contact points between type 2 and VGluT3-positive amacrine cells (I). J, Colocalized points from all three channels are highlighted in magenta and projected onto the type 2 cell terminal (arrowheads). K, L, Pixel intensity plot for the area marked by dashed lines in I. Pixel intensity was larger than threshold (for details, see Materials and Methods) and was normalized and plotted against distance. Following Bolte and Cordelières (2006), we defined colocalization between the type 2 cell dendrite (green) and the glycine receptor (red) as when the true overlap distance (arrow) of the normalized fluorescence intensity curves at mid-height (dashed line) was larger than ∼245 nm (lateral resolution of the 63× objective). For details, see Materials and Methods. Overlap distances of 418 and 254 nm, respectively, confirmed the presence of glycine receptors at contact points between a glycinergic amacrine cell and GFP-positive type 2 cell terminals. Please note that intensity values for VGluT3 are only shown to illustrate the spatial relationship between all three stainings. Scale bars: A–J, 10 μm.

Article Snippet: VGluT3 , Synthetic peptide from rat VGluT3 protein , Millipore, AB5421 , 1:5000, guinea pig, polyclonal.

Techniques: Labeling, Marker, Fluorescence

Journal: The Journal of Neuroscience

Article Title: Inputs Underlying the ON–OFF Light Responses of Type 2 Wide-Field Amacrine Cells in TH::GFP Mice

doi: 10.1523/JNEUROSCI.6235-10.2011

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: VGluT3 , Synthetic peptide from rat VGluT3 protein , Millipore, AB5421 , 1:5000, guinea pig, polyclonal.

Techniques: Sequencing, Recombinant, Binding Assay

Journal: The Journal of Neuroscience

Article Title: Inputs Underlying the ON–OFF Light Responses of Type 2 Wide-Field Amacrine Cells in TH::GFP Mice

doi: 10.1523/JNEUROSCI.6235-10.2011

Figure Lengend Snippet: Type 2 cells receive inputs from VGluT3-immunoreactive amacrine cells and express glycine receptors. A–D, Single scans (0.2 μm) of a retina section from a TH::GFP mouse labeled with antibodies against VGluT3, a marker for a certain type of glycinergic amacrine cell (B), and the glycine receptor 2α subunit (C). D, Merged image. E, Projection of 15 scans (3 μm). F–J, Single confocal scans show the area marked in D under higher magnification. GFP-positive dendrites from type 2 cells (E) are contacted by VGluT3-positive dendrites (G). GlyR2α-positive puncta (H) are visible at contact points between type 2 and VGluT3-positive amacrine cells (I). J, Colocalized points from all three channels are highlighted in magenta and projected onto the type 2 cell terminal (arrowheads). K, L, Pixel intensity plot for the area marked by dashed lines in I. Pixel intensity was larger than threshold (for details, see Materials and Methods) and was normalized and plotted against distance. Following Bolte and Cordelières (2006), we defined colocalization between the type 2 cell dendrite (green) and the glycine receptor (red) as when the true overlap distance (arrow) of the normalized fluorescence intensity curves at mid-height (dashed line) was larger than ∼245 nm (lateral resolution of the 63× objective). For details, see Materials and Methods. Overlap distances of 418 and 254 nm, respectively, confirmed the presence of glycine receptors at contact points between a glycinergic amacrine cell and GFP-positive type 2 cell terminals. Please note that intensity values for VGluT3 are only shown to illustrate the spatial relationship between all three stainings. Scale bars: A–J, 10 μm.

Article Snippet: VGluT3 , Synthetic peptide from rat VGluT3 protein , Millipore, AB5421 , 1:5000, guinea pig, polyclonal.

Techniques: Labeling, Marker, Fluorescence